M2 medium

M2 medium

sterility:sterile-filtered.

form:liquid.

storage condition:protect from light.

technique(s):cell culture | embryo: suitable.

impurities:endotoxin, tested.

components:phenol red: 0.0106 g/L;sodium pyruvate: 0.0363 g/L;HEPES: 5.42726 g/L;glucose: 1.0 g/L (Dextro);NaHCO3: 0.35 g/L.Application:

Recommended for manipulation of mouse embryos at ambient temperature. M2 and M16 Medium are common media for in vitro culture of preimplantation stage embryos. It is a modified Krebs-Ringer bicarbonate solution, which is very similar to Whitten′s Medium. M16 contains pyruvate and lactate as energy sources since preimplantation embryos do not utilize glucose efficiently.

M2 Medium is a further modification of M16 that substitutes HEPES buffer in place of some of the bicarbonate. M2 is used for collecting and handling embryos for prolonged periods outside a CO2 incubator.